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Nat Biotechnol. 2002 Aug;20(8):795-9. Epub 2002 Jul 22.

Cloned zebrafish by nuclear transfer from long-term-cultured cells.

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  • 1Department of Molecular, Cellular, and Developmental Biology, University of California Los Angeles, Los Angeles, CA 90095-1606, USA.

Abstract

Although mammals have been cloned from genetically manipulated cultured cells, a comparable achievement has not been realized in lower vertebrates. Here we report that fertile transgenic zebrafish can be obtained by nuclear transfer using embryonic fibroblast cells from long-term cultures. The donor nuclei, modified by retroviral insertions expressing green fluorescent protein (GFP), were transplanted into manually enucleated eggs. Overall, a 2% success rate was achieved, resulting in 11 adult transgenic zebrafish expressing GFP. These nuclear transplants produced fertile, diploid offspring, and their F1/F2 progeny continued to express GFP in a pattern identical to that of the founder fish. This finding demonstrates that slowly dividing nuclei from cultured cells can be reprogrammed to support rapid embryonic development and sets up a foundation for targeted genetic manipulation in zebrafish.

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  • Attack of the fish clones. [Nat Biotechnol. 2002]
PMID:
12134167
[PubMed - indexed for MEDLINE]
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