Abstract
A commercially available filtration method is described for the purification of polymerase chain reaction (PCR) templates and sequence-labeled products. The methodology is described for the automation of this application and its use on a high-throughput liquid handling robot and capillary-based automated DNA sequencer. The application provides good-quality DNA, is relatively cheap, and can be used in either 96- or 384-well format.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Typing Techniques
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Electrophoresis / methods
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Humans
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Micropore Filters*
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Neisseria meningitidis / classification
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Neisseria meningitidis / genetics
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Polymerase Chain Reaction / instrumentation
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Polymerase Chain Reaction / methods*
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Robotics*
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Sequence Analysis, DNA*
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Streptococcus pneumoniae / classification
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Streptococcus pneumoniae / genetics
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Templates, Genetic*