Automated PCR/sequence template purification

Mol Biotechnol. 2002 Jul;21(3):221-4. doi: 10.1385/MB:21:3:221.

Abstract

A commercially available filtration method is described for the purification of polymerase chain reaction (PCR) templates and sequence-labeled products. The methodology is described for the automation of this application and its use on a high-throughput liquid handling robot and capillary-based automated DNA sequencer. The application provides good-quality DNA, is relatively cheap, and can be used in either 96- or 384-well format.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Electrophoresis / methods
  • Humans
  • Micropore Filters*
  • Neisseria meningitidis / classification
  • Neisseria meningitidis / genetics
  • Polymerase Chain Reaction / instrumentation
  • Polymerase Chain Reaction / methods*
  • Robotics*
  • Sequence Analysis, DNA*
  • Streptococcus pneumoniae / classification
  • Streptococcus pneumoniae / genetics
  • Templates, Genetic*