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Cell Calcium. 2002 May;31(5):245-51.

Measuring zinc in living cells. A new generation of sensitive and selective fluorescent probes.

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  • 1Molecular Probes, Inc., 4849 Pitchford Avenue, Eugene, OR 97478, USA.


New fluorescent indicators with nanomolar to micromolar affinities for Zn(2+) have been synthesized in wavelengths from UV to the far red. The UV light-excited indicators are ratiometric. The visible wavelength indicators are non-ratiometric and exhibit large and pH-independent fluorescence increases with increasing zinc concentrations, with little to no sensitivity to physiologically relevant Ca(2+) concentrations. Experiments in neuronal and non-neuronal cell cultures show the new indicators to retain their sensitivity to and selectivity for zinc after conversion to cell-permeable forms.

(c) 2002 Elsevier Science Ltd. All rights reserved.

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