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Clin Microbiol Rev. 2002 Jul;15(3):329-41.

Methods for cultivation of luminal parasitic protists of clinical importance.

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  • 1Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, Great Britain. graham.clark@lshtm.ac.uk


Cultivation of luminal protistan parasites has a long history. In this review we discuss the methods and media that are most widely used for the establishment and maintenance of the following organisms in culture: Entamoeba histolytica, Giardia intestinalis, Trichomonas vaginalis, Dientamoeba fragilis, Blastocystis hominis, and Balantidium coli. While cultivation is of limited importance in the diagnostic laboratory, it is essential to most research laboratories, and it is toward the latter that this review is primarily aimed.

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