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Science. 2002 Jun 28;296(5577):2395-8.

Channelrhodopsin-1: a light-gated proton channel in green algae.

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  • 1Max-Planck-Institut für Biophysik, Kennedyallee 70, 60596 Frankfurt am Main, Germany. nagel@mpibp-frankfurt.mpg.de

Abstract

Phototaxis and photophobic responses of green algae are mediated by rhodopsins with microbial-type chromophores. We report a complementary DNA sequence in the green alga Chlamydomonas reinhardtii that encodes a microbial opsin-related protein, which we term Channelopsin-1. The hydrophobic core region of the protein shows homology to the light-activated proton pump bacteriorhodopsin. Expression of Channelopsin-1, or only the hydrophobic core, in Xenopus laevis oocytes in the presence of all-trans retinal produces a light-gated conductance that shows characteristics of a channel selectively permeable for protons. We suggest that Channelrhodopsins are involved in phototaxis of green algae.

PMID:
12089443
[PubMed - indexed for MEDLINE]
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