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J Bacteriol. 2002 Jul;184(14):3931-40.

Novel DNA-binding proteins in the cyanobacterium Anabaena sp. strain PCC 7120.

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  • 1MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing 48824, USA.

Abstract

As an approach towards elucidation of the biochemical regulation of the progression of heterocyst differentiation in Anabaena sp. strain PCC 7120, we have identified proteins that bind to a 150-bp sequence upstream from hepC, a gene that plays a role in the synthesis of heterocyst envelope polysaccharide. Such proteins were purified in four steps from extracts of vegetative cells of Anabaena sp. Two of these proteins (Abp1 and Abp2) are encoded by neighboring genes in the Anabaena sp. chromosome. The genes that encode the third (Abp3) and fourth (Abp4) proteins are situated at two other loci in that chromosome. Insertional mutagenesis of abp2 and abp3 blocked expression of hepC and hepA and prevented heterocyst maturation and aerobic fixation of N(2).

PMID:
12081965
PMCID:
PMC135189
[PubMed - indexed for MEDLINE]
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