Public opinion and official bodies place great emphasis on the reduction, refinement and replacement of the use of laboratory animals in testing protocols. In the field of toxicology, major efforts are being made to commit to this goal. The testing of reproductive function is currently still performed by in-vivo tests, mainly in rodents. In the past, follicle culture models were developed for the in-vitro production of mature oocytes and used to study the process of folliculogenesis and oogenesis in vitro. These culture systems might be able to acquire a place in fertility testing, replacing in-vivo studies for ovarian function and female gamete quality testing. The proficiency data from a well-characterized follicle culture system suggest that this bioassay might be of potential use for in-vitro screening of xenobiotic substances affecting ovarian function and fertility.