Co-expressions of different opioid receptor types differentially modulate their signaling via G(16)

Neurosignals. 2002 Mar-Apr;11(2):115-22. doi: 10.1159/000058548.

Abstract

Combinations of two different types of opioid receptors - delta-, kappa-, mu-opioid receptors (DOR, KOR, and MOR) and opioid receptor-like receptor 1 (ORL(1)) - were co-expressed with the alpha subunit of G(16) in COS-7 cells, and the ability of various selective agonists to induce activation of phospholipase Cbeta was examined. Nociceptin/orphanin FQ-induced response was enhanced when ORL(1) was co-expressed with MOR or KOR but not DOR. The kappa-agonist U50,488H induced a modest inositol phosphate formation when KOR was expressed alone or with MOR, but the response was attenuated when co-expressing with either DOR or ORL(1). It is suggested that the co-expressions of two different opioid receptor types indeed modify their downstream signaling events.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer / pharmacology
  • Analgesics / pharmacology
  • Analgesics, Non-Narcotic / pharmacology
  • Animals
  • Benzeneacetamides*
  • COS Cells
  • GTP-Binding Protein alpha Subunits, Gq-G11
  • Gene Expression / physiology
  • Heterotrimeric GTP-Binding Proteins / metabolism
  • Isoenzymes / metabolism
  • Nociceptin
  • Nociceptin Receptor
  • Opioid Peptides / pharmacology
  • Phospholipase C beta
  • Protein Binding / drug effects
  • Protein Binding / physiology
  • Pyrrolidines / pharmacology
  • Receptors, Opioid / genetics*
  • Receptors, Opioid / metabolism
  • Receptors, Opioid, delta / genetics
  • Receptors, Opioid, delta / metabolism
  • Receptors, Opioid, kappa / genetics
  • Receptors, Opioid, kappa / metabolism
  • Receptors, Opioid, mu / genetics
  • Receptors, Opioid, mu / metabolism
  • Signal Transduction / physiology*
  • Type C Phospholipases / metabolism

Substances

  • Analgesics
  • Analgesics, Non-Narcotic
  • Benzeneacetamides
  • Isoenzymes
  • Opioid Peptides
  • Pyrrolidines
  • Receptors, Opioid
  • Receptors, Opioid, delta
  • Receptors, Opioid, kappa
  • Receptors, Opioid, mu
  • 3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer
  • Type C Phospholipases
  • Phospholipase C beta
  • G protein alpha 16
  • GTP-Binding Protein alpha Subunits, Gq-G11
  • Heterotrimeric GTP-Binding Proteins
  • U 69593
  • Nociceptin Receptor