Abstract
Combinations of two different types of opioid receptors - delta-, kappa-, mu-opioid receptors (DOR, KOR, and MOR) and opioid receptor-like receptor 1 (ORL(1)) - were co-expressed with the alpha subunit of G(16) in COS-7 cells, and the ability of various selective agonists to induce activation of phospholipase Cbeta was examined. Nociceptin/orphanin FQ-induced response was enhanced when ORL(1) was co-expressed with MOR or KOR but not DOR. The kappa-agonist U50,488H induced a modest inositol phosphate formation when KOR was expressed alone or with MOR, but the response was attenuated when co-expressing with either DOR or ORL(1). It is suggested that the co-expressions of two different opioid receptor types indeed modify their downstream signaling events.
Copyright 2002 S. Karger AG, Basel
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer / pharmacology
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Analgesics / pharmacology
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Analgesics, Non-Narcotic / pharmacology
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Animals
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Benzeneacetamides*
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COS Cells
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GTP-Binding Protein alpha Subunits, Gq-G11
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Gene Expression / physiology
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Heterotrimeric GTP-Binding Proteins / metabolism
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Isoenzymes / metabolism
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Nociceptin
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Nociceptin Receptor
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Opioid Peptides / pharmacology
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Phospholipase C beta
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Protein Binding / drug effects
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Protein Binding / physiology
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Pyrrolidines / pharmacology
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Receptors, Opioid / genetics*
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Receptors, Opioid / metabolism
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Receptors, Opioid, delta / genetics
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Receptors, Opioid, delta / metabolism
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Receptors, Opioid, kappa / genetics
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Receptors, Opioid, kappa / metabolism
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Receptors, Opioid, mu / genetics
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Receptors, Opioid, mu / metabolism
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Signal Transduction / physiology*
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Type C Phospholipases / metabolism
Substances
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Analgesics
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Analgesics, Non-Narcotic
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Benzeneacetamides
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Isoenzymes
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Opioid Peptides
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Pyrrolidines
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Receptors, Opioid
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Receptors, Opioid, delta
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Receptors, Opioid, kappa
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Receptors, Opioid, mu
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3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer
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Type C Phospholipases
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Phospholipase C beta
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G protein alpha 16
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GTP-Binding Protein alpha Subunits, Gq-G11
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Heterotrimeric GTP-Binding Proteins
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U 69593
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Nociceptin Receptor