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Acta Crystallogr D Biol Crystallogr. 2002 Jul;58(Pt 7):1201-3. Epub 2002 Jun 20.

Purification and initial crystallization studies of a DnaB intein from Synechocystis sp. PCC 6803.

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  • 1Laboratory of Structural Biology, Department of Biological Science and Technology and MOE Laboratory of Protein Science, Tsinghua University, Beijing 100084, People's Republic of China.


A 154-residue mini-intein from the dnaB gene of Synechocystis sp. PCC 6803 (Ssp DnaB intein) has been purified and crystallized using PEG 4000 as a precipitant. The crystal belongs to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 58.2, c = 70.3 A. It has one molecule per asymmetric unit and diffracts to beyond 2.0 A under cryoconditions (100 K) using a rotating copper anode X-ray generator.

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