Format

Send to:

Choose Destination
See comment in PubMed Commons below
J Virol. 2002 Jul;76(14):7040-8.

Translational regulation of human papillomavirus type 16 E7 mRNA by the peptide SEQIKA, shared by rabbit alpha(1)-globin and human cytokeratin 7.

Author information

  • 1CARSO Cancer Research Center, Valenzano, Regione Puglia, Italy. d.kanduc@biologia.uniba.it

Abstract

The possible biochemical factors able to affect the in vitro expression of the high-risk human papillomavirus type 16 (HPV16) E7 oncoprotein have been analyzed. Evidence is provided that E7 mRNA stability is increased and, conversely, transcript translation is inhibited by binding to a 32-kDa protein from rabbit reticulocyte lysate; sequence analysis identified the 32-kDa binding protein as rabbit alpha(1)-globin protein; and interaction between rabbit alpha(1)-globin and E7 mRNA occurs through the 6-mer peptide SEQIKA present in human cytokeratin 7 protein. The in vitro data were confirmed by the occurrence of HPV16 E7 mRNA-cytokeratin 7 binding in squamous cervical cancer SiHa cells.

PMID:
12072504
[PubMed - indexed for MEDLINE]
PMCID:
PMC136328
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk