Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Plant Physiol. 2002 Jun;129(2):440-50.

Arabidopsis map-based cloning in the post-genome era.

Author information

  • 1Cereon Genomics LLC, 45 Sidney Street, Cambridge, MA 02139, USA. georg.jander@cereon.com

Abstract

Map-based cloning is an iterative approach that identifies the underlying genetic cause of a mutant phenotype. The major strength of this approach is the ability to tap into a nearly unlimited resource of natural and induced genetic variation without prior assumptions or knowledge of specific genes. One begins with an interesting mutant and allows plant biology to reveal what gene or genes are involved. Three major advances in the past 2 years have made map-based cloning in Arabidopsis fairly routine: sequencing of the Arabidopsis genome, the availability of more than 50,000 markers in the Cereon Arabidopsis Polymorphism Collection, and improvements in the methods used for detecting DNA polymorphisms. Here, we describe the Cereon Collection and show how it can be used in a generic approach to mutation mapping in Arabidopsis. We present the map-based cloning of the VTC2 gene as a specific example of this approach.

PMID:
12068090
[PubMed - indexed for MEDLINE]
PMCID:
PMC1540230
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk