Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
J Chromatogr A. 2002 Apr 5;952(1-2):149-63.

Liquid exclusion-adsorption chromatography, a new technique for isocratic separation of nonionic surfactants. IV. Two-dimensional separation of fatty alcohol ethoxylates with focusing of fractions.

Author information

  • 1Institute of Chemistry, Karl-Franzens-University Graz, Austria. trathnig@kfunigraz.ac.at

Abstract

Fatty alcohol ethoxylates can be analyzed using a combination of liquid chromatography under critical conditions as the first dimension and liquid exclusion-adsorption chromatography as the second dimension. Transfer of fractions from the first to the second dimension is achieved using the full adsorption-desorption (FAD) technique. The peaks of interest in the first dimension are trapped on a short precolumn before injecting them into the second dimension. Full adsorption is achieved by increasing the water content in the mobile phase before the FAD column. When the fractions are desorbed by switching to the mobile phase of the second dimension, they are focused and reconcentrated. In this way, a full resolution of oligomers is achieved. As both dimensions are run in isocratic mode, density and refractive index detection can be applied, which allows an accurate quantitation.

PMID:
12064527
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Write to the Help Desk