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J Chromatogr A. 2002 Apr 5;952(1-2):149-63.

Liquid exclusion-adsorption chromatography, a new technique for isocratic separation of nonionic surfactants. IV. Two-dimensional separation of fatty alcohol ethoxylates with focusing of fractions.

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  • 1Institute of Chemistry, Karl-Franzens-University Graz, Austria. trathnig@kfunigraz.ac.at


Fatty alcohol ethoxylates can be analyzed using a combination of liquid chromatography under critical conditions as the first dimension and liquid exclusion-adsorption chromatography as the second dimension. Transfer of fractions from the first to the second dimension is achieved using the full adsorption-desorption (FAD) technique. The peaks of interest in the first dimension are trapped on a short precolumn before injecting them into the second dimension. Full adsorption is achieved by increasing the water content in the mobile phase before the FAD column. When the fractions are desorbed by switching to the mobile phase of the second dimension, they are focused and reconcentrated. In this way, a full resolution of oligomers is achieved. As both dimensions are run in isocratic mode, density and refractive index detection can be applied, which allows an accurate quantitation.

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