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Dev Cell. 2002 Jun;2(6):819-30.

miwi, a murine homolog of piwi, encodes a cytoplasmic protein essential for spermatogenesis.

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  • 1Department of Cell Biology, Duke University Medical Center, P.O. Box 3709, DUMC, Durham, NC 27710, USA.

Abstract

The piwi family genes are crucial for stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. However, their function in mammals remains unexplored. Here we report the cloning of a murine piwi gene (miwi) essential for spermatogenesis. miwi encodes a cytoplasmic protein specifically expressed in spermatocytes and spermatids. miwi(null) mice display spermatogenic arrest at the beginning of the round spermatid stage, resembling the phenotype of CREM, a master regulator of spermiogenesis. Furthermore, mRNAs of ACT (activator of CREM in testis) and CREM target genes are downregulated in miwi(null) testes. Whereas MIWI and CREM do not regulate each other's expression, MIWI complexes with mRNAs of ACT and CREM target genes. Hence, MIWI may control spermiogenesis by regulating the stability of these mRNAs.

PMID:
12062093
[PubMed - indexed for MEDLINE]
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