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Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8695-700. Epub 2002 Jun 11.

Methylation of histone H3 Lys 4 in coding regions of active genes.

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  • 1Department of Chemistry and Chemical Biology and The Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA.

Abstract

Posttranslational modifications of histone tails regulate chromatin structure and transcription. Here we present global analyses of histone acetylation and histone H3 Lys 4 methylation patterns in yeast. We observe a significant correlation between acetylation of histones H3 and H4 in promoter regions and transcriptional activity. In contrast, we find that dimethylation of histone H3 Lys 4 in coding regions correlates with transcriptional activity. The histone methyltransferase Set1 is required to maintain expression of these active, promoter-acetylated, and coding region-methylated genes. Global comparisons reveal that genomic regions deacetylated by the yeast enzymes Rpd3 and Hda1 overlap extensively with Lys 4 hypo- but not hypermethylated regions. In the context of recent studies showing that Lys 4 methylation precludes histone deacetylase recruitment, we conclude that Set1 facilitates transcription, in part, by protecting active coding regions from deacetylation.

PMID:
12060701
[PubMed - indexed for MEDLINE]
PMCID:
PMC124361
Free PMC Article

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