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Science. 2002 Jun 7;296(5574):1876-80.

Dynamics of thymocyte-stromal cell interactions visualized by two-photon microscopy.

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  • 1Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.

Abstract

Thymocytes are selected to mature according to their ability to interact with self major histocompatibility complex (MHC)-peptide complexes displayed on the thymic stroma. Using two-photon microscopy, we performed real-time analysis of the cellular contacts made by developing thymocytes undergoing positive selection in a three-dimensional thymic organ culture. A large fraction of thymocytes within these cultures were highly motile. MHC recognition was found to increase the duration of thymocyte-stromal cell interactions and occurred as both long-lived cellular associations displaying stable cell-cell contacts and as shorter, highly dynamic contacts. Our results identify the diversity and dynamics of thymocyte interactions during positive selection.

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PMID:
12052962
[PubMed - indexed for MEDLINE]
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