Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Science. 2002 May 24;296(5572):1459-62.

Pyrrolysine encoded by UAG in Archaea: charging of a UAG-decoding specialized tRNA.

Author information

  • 1Department of Microbiology, Ohio State University, Columbus, OH 43210, USA.

Abstract

Pyrrolysine is a lysine derivative encoded by the UAG codon in methylamine methyltransferase genes of Methanosarcina barkeri. Near a methyltransferase gene cluster is the pylT gene, which encodes an unusual transfer RNA (tRNA) with a CUA anticodon. The adjacent pylS gene encodes a class II aminoacyl-tRNA synthetase that charges the pylT-derived tRNA with lysine but is not closely related to known lysyl-tRNA synthetases. Homologs of pylS and pylT are found in a Gram-positive bacterium. Charging a tRNA(CUA) with lysine is a likely first step in translating UAG amber codons as pyrrolysine in certain methanogens. Our results indicate that pyrrolysine is the 22nd genetically encoded natural amino acid.

Comment in

PMID:
12029131
[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for HighWire Icon for Faculty of 1000
    Loading ...
    Write to the Help Desk