Abstract
Ovulation in Caenorhabditis elegans requires inositol 1,4,5-triphosphate (IP(3)) signaling activated by the epidermal growth factor (EGF)-receptor homolog LET-23. We generated a deletion mutant of a type I 5-phosphatase, ipp-5, and found a novel ovulation phenotype whereby the spermatheca hyperextends to engulf two oocytes per ovulation cycle. The temporal and spatial expression of IPP-5 is consistent with its proposed inhibition of IP(3) signaling in the adult spermatheca. ipp-5 acts downstream of let-23, and interacts with let-23-mediated IP(3) signaling pathway genes. We infer that IPP-5 negatively regulates IP(3) signaling to ensure proper spermathecal contraction.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Caenorhabditis elegans / enzymology
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Caenorhabditis elegans / genetics
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Caenorhabditis elegans / metabolism*
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Caenorhabditis elegans Proteins*
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Epidermal Growth Factor*
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ErbB Receptors / metabolism
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Helminth Proteins / metabolism
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Inositol 1,4,5-Trisphosphate / metabolism*
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Inositol Polyphosphate 5-Phosphatases
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Molecular Sequence Data
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Ovulation / metabolism*
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Phosphoric Monoester Hydrolases / genetics
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Phosphoric Monoester Hydrolases / metabolism*
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Sequence Alignment
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Sequence Analysis, DNA
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Sequence Deletion
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Signal Transduction* / physiology
Substances
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Caenorhabditis elegans Proteins
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Helminth Proteins
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Lin-3 protein, C elegans
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Epidermal Growth Factor
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Inositol 1,4,5-Trisphosphate
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ErbB Receptors
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let-23 protein, C elegans
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Phosphoric Monoester Hydrolases
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Inositol Polyphosphate 5-Phosphatases