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Clin Exp Allergy. 2002 Jan;32(1):93-6.

New polymorphisms of haematopoietic prostaglandin D synthase and human prostanoid DP receptor genes.

Author information

  • 1Department of Medical Genetics, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba City, Japan. mo00f210@md.tsukuba.ac.jp

Abstract

BACKGROUND:

Prostaglandin D2 (PGD2), a major cyclo-oxygenase metabolite of arachidonic acid in mast cells, induces bronchoconstriction in the human lung. It has been reported that mice lacking PGD receptor fail to develop the bronchial hyper-responsiveness upon ovalbumin challenge, suggesting that PGD2 functions as a mediator of allergic asthma.

OBJECTIVE:

To determine if there are any mutations associated with the development of asthma in the haematopoietic prostaglandin D synthase (H-PGDS) gene and the human prostanoid DP receptor (PTGDR) gene.

METHODS AND RESULTS:

We screened the 5'flanking and coding regions of the H-PGDS gene and the PTGDR gene by direct sequence. We identified one variant in intron 2 (IVS2 + 11 A > C) and one variant in intron 3 (IVS3 + 13T > C) of the H-PGDS gene, and two variants in the 5'flanking region of the PTGDR gene (-197T > C and -2C > T). The IVS3 + 13T > C and -197T > C variants were rare, appearing only once in 48 subjects. transmission disequilibrium test (TDT) analysis of 144 asthmatic families revealed that the IVS2 + 11 A allele of the H-PGDS gene was significantly transmitted preferentially to asthma-affected children (P = 0.0056), but no association was observed between -2C/T polymorphism of the PTGDR gene and asthma (P > 0.05).

CONCLUSION:

Our results suggest that the IVS2 + 11A/C allele may be involved in the development of asthma in the Japanese population.

PMID:
12002745
[PubMed - indexed for MEDLINE]
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