FEBS Lett. 1983 Apr 5;154(1):127-33.

Kinetics of 5-enolpyruvylshikimate-3-phosphate synthase inhibition by glyphosate.

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Department of Biochemistry, University of Glasgow, Scotland.

Abstract

The herbicide glyphosate (N-phosphonomethyl glycine) is a potent reversible inhibitor of the 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase activity of the purified arom multienzyme complex from Neurospora crassa. Inhibition of the EPSP synthase reaction by glyphosate is competitive with respect to phosphoenolpyruvate, with K(i) 1.1 microM, and uncompetitive with respect to shikimate-3-phosphate. The kinetic patterns are consistent with a compulsory order sequential mechanism in which either PEP or glyphosate can bind to an enzyme: shikimate-3-phosphate complex.

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Cited by 19 PubMed Central articles

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