FEBS Lett. 2002 Mar 13;514(2-3):243-9.

Crystal structure of plant pectin methylesterase.

Johansson K, El-Ahmad M, Friemann R, Jörnvall H, Markovic O, Eklund H.

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Department of Molecular Biology, Swedish University of Agricultural Sciences, S-751 24 Uppsala, Sweden.

Abstract

Pectin is a principal component in the primary cell wall of plants. During cell development, pectin is modified by pectin methylesterases to give different properties to the cell wall. This report describes the first crystal structure of a plant pectin methylesterase. The beta-helical structure embodies a central cleft, lined by several aromatic residues, that has been deduced to be suitable for pectin binding. The active site is found at the center of this cleft where Asp157 is suggested to act as the nucleophile, Asp136 as an acid/base and Gln113/Gln135 to form an anion hole to stabilize the transition state.

PMID:: 11943159; [PubMed - indexed for MEDLINE]

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Research Support, Non-U.S. Gov't

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Secondary Source ID

PDB/1GQ8

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Cited by 10 PubMed Central articles

Isolation and Expression analysis of OsPME1, encoding for a putative Pectin Methyl Esterase from Oryza sativa (subsp. indica). [Physiol Mol Biol Plants. 2009]
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Rheo-NMR studies of an enzymatic reaction: evidence of a shear-stable macromolecular system. [Biophys J. 2010]
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Structures reported by this article

Pectin Methylesterase From Carrot

PDB: 1GQ8

Source: Daucus carota

Method: X-Ray Diffraction

Resolution: 1.75 Å

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