My NCBISign In

Display Settings:

Format

Send to:

Choose Destination
    Hunan Yi Ke Da Xue Xue Bao. 1999;24(2):119-22.

    [Methodological study on the assay of glycosylphosphatidylinositol-specific phospholipase D activity in serum].

    [Article in Chinese]
    Tang J, Li W.

    Source

    Department of Biochemistry, Hunan Medical University, Changsha 410078.

    Abstract

    A method for the assay of glycosylphosphatidylinositol-specific phospholipase D(GPI-PLD) activity in human serum was established by using glycosylphosphatidylinositol(GPI) anchored placental alkaline phosphatase(PLAP) as a substrate. Serum GPI-PLD activity was determined both qualitatively and quantitatively by PEG/dextran and triton-X-114 partitioning respectively, and some kinetic properties of its enzymatic reaction were also studied. This method revealed a satisfactory reproductivity(CV = 3.6%) and a high sensitivity(2 microliters serum was sufficient). In addition, it is practical, economical, and feasible to be widely applied. The activities of GPI-PLD measured by this method in 100 normal subjects ranged from 30% to 50% (in terms of the percentage of converting GPI anchored PLAP substrate to the anchor-free product).

    PMID:
    11938765
    [PubMed - indexed for MEDLINE]

    Publication Types, MeSH Terms, Substances

    Publication Types

    MeSH Terms

    Substances

      Supplemental Content

      Save items

      loading

      Related citations in PubMed

      See reviews... See all...

      Related information

      • Related Citations
        Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
      • Gene
        Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
      • Gene (GeneRIF)
        Gene records that have the current articles as Reference into Function citations (GeneRIFs). NLM staff reviewing the literature while indexing MEDLINE add GeneRIFs manually.
      • HomoloGene
        HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
      • Nucleotide (RefSeq)
        NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
      • Nucleotide (Weighted)
        Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
      • Protein (RefSeq)
        NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
      • Protein (Weighted)
        Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
      • Substance (MeSH Keyword)
        PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
      • Taxonomy via GenBank
        Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
      • UniGene
        UniGene clusters of expressed sequences that are associated with the current articles through references on the clustered sequence records and related Gene records.
      • GEO Profiles
        Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.

      Recent activity

      Clear Turn Off Turn On

      Your browsing activity is empty.

      Activity recording is turned off.

      Turn recording back on

      See more...
      You are here: NCBI > Literature > PubMed
      Write to the Help Desk