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Mol Biotechnol. 2002 Mar;20(3):231-7.

Magnetite-alginate beads for purification of some starch degrading enzymes.

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  • 1Chemistry Department, Indian Institute of Technology, Hauz Khas, New Delhi.

Abstract

Starch degrading enzymes, viz., beta-amylase, glucoamylase, and pullulanase, were purified using magnetite-alginate beads. In each case, the enzyme activity was eluted by using 1.0 M maltose. beta-Amylase (sweet potato), glucoamylase (Aspergillus niger), and pullulanase (Bacillus acidopullulyticus) from their crude preparations were purified 37-, 31-, and 49-fold with 86, 87, and 95% activity recovery, respectively. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed single band in each case.

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