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Exp Cell Res. 2002 Apr 1;274(2):246-53.

Hsp70 family member, mot-2/mthsp70/GRP75, binds to the cytoplasmic sequestration domain of the p53 protein.

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  • 1Chugai Research Institute for Medical Sciences, 153-2 Nagai, Niihari Mura, Ibaraki, 300-41, Japan.

Abstract

Hsp70 family member mot-2/mthsp70/GRP75/PBP74 was shown to bind to the tumor suppressor protein p53. In this study, by in vivo coimmunoprecipitation of mot-2 with p53 and its deletion mutants, the mot-2 binding site of p53 was mapped to its C-terminal amino acid residues 312-352, a region of p53 that includes its cytoplasmic sequestration domain. These data demonstrate that cytoplasmic sequestration and inactivation of p53 by mot-2 occurs by its binding to the cytoplasmic sequestration domain. Therefore, perturbation of mot-p53 interactions can be employed to abrogate cytoplasmic retention of wild-type p53 in tumors.

Copyright 2002 Elsevier Science (USA).

PMID:
11900485
[PubMed - indexed for MEDLINE]
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