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1: Infect Immun. 2002 Apr;70(4):2238-41.Click here to read Click here to read Links

Gel shift assay of nuclear extracts from Histoplasma capsulatum demonstrates the presence of several DNA binding proteins.

Ignatov A, Keath EJ.

Department of Biology, Saint Louis University, St. Louis, Missouri 63103, USA.

A gel shift assay was optimized to detect several general DNA binding proteins from Histoplasma capsulatum strain G217B. The electrophoretic mobility shift assay (EMSA) technique also detected protein(s) recognizing a pyrimidine-rich motif found in several Histoplasma promoters. Establishment of EMSA conditions provides an important framework to evaluate regulation of homeostatic or phase-specific genes that may influence virulence in Histoplasma and other dimorphic fungal pathogens.

PMID: 11895995 [PubMed - indexed for MEDLINE]

PMCID: PMC127865