Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Arch Biochem Biophys. 2002 Mar 15;399(2):212-24.

Identification of novel E2F1-regulated genes by microarray.

Author information

  • 1Program in Molecular Oncology, H. Lee Moffitt Comprehensive Cancer Center and Research Institute, University of South Florida, Tampa, 33612, USA.

Abstract

The E2F pathway has been proposed to regulate genes involved in the transition from quiescence into DNA synthesis. However, this hypothesis has not been rigorously tested on a genomic scale. Toward this end, we have infected quiescent mouse fibroblasts, which do not express E2F1, with an E2F1-expressing adenovirus and examined the expression of more than 6000 genes using high-density microarrays. Microarray results clearly support the current paradigm; however, they suggest that E2F1 may also regulate unanticipated cellular functions including pathways involved in apoptosis, signal transduction, transcriptional control, and membrane biology. Most surprisingly, we identified a number of genes that are repressed by E2F1 expression, suggesting that E2F1 may have the potential to repress transcription of numerous genes through an unknown mechanism.

PMID:
11888208
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk