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Mol Microbiol. 2001 Dec;42(5):1311-23.

A molecular mechanism for the repression of transcription by the H-NS protein.

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  • 1UnitĂ© de Physicochimie des MacromolĂ©cules Biologiques, URA 1773 du Centre National de la Recherche Scientifique, Institut Pasteur, F-75724 Paris Cedex 15, France. srimsky@igr.fr


The H-NS protein is a major component of the bacterial nucleoid and plays a crucial role in the global gene regulation of enteric bacteria. Although H-NS does not exhibit a high DNA sequence specificity, a number of H-NS-responsive promoters have been shown to contain regions of intrinsic DNA curvature located either upstream or downstream of the transcription start point. We have studied H-NS binding to DNA and in vitro transcriptional regulation by H-NS at several synthetic promoters with or without curved sequences inserted upstream of the Pribnow box. We show how such inserts determine the final organization of H-NS-containing nucleoprotein complexes and how this affects transcription. We refine a two-step mechanism for the constitution of H-NS assemblies that are efficient in regulation.

[PubMed - indexed for MEDLINE]
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