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J Am Chem Soc. 2002 Mar 13;124(10):2120-1.

Electrical contacting of glucose oxidase by surface-reconstitution of the apo-protein on a relay-boronic acid-FAD cofactor monolayer.

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  • 1Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.


A new methodology for the surface-reconstitution of apo-flavoenzymes on a relay unit (pyrroloquinoline quinone, PQQ) functionalized with a boronic acid ligand as a linker to native FAD was developed. The reconstitution of apo-glucose oxidase (apo-GOx) on the PQQ-FAD monolayer yields an electrically contacted enzyme-electrode.

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