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Syst Appl Microbiol. 2001 Dec;24(4):634-8.

Yeasts present during wine fermentation: comparative analysis of conventional plating and PCR-TTGE.

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  • 1Facultad de Ciencias Químicas, Universidad de Castilla La Mancha, Ciudad Real, Spain.


Yeasts isolated from must before and during fermentation at a wine cellar of La Mancha region in Spain were characterised using Polymerase Chain Reaction / Restriction Fragments Lengths Polymorphism and Polymerase Chain Reaction / Temporal Temperature Gradient Gel Electrophoresis. S. cerevisiae strains were differentiated using mtDNA restriction analysis. Direct PCR-TTGE was also used to study biodiversity during wine fermentation, and revealed the variations in the population. It was observed that isolation by conventional plating may afford a skewed view of the strains taking part in wine fermentation.

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