Abstract
S100B, a glia-derived calcium binding protein, exhibits strong neurite extension activity in cultured serotonergic neurons. Using S100B-knockout mice, we examined whether this protein possesses in vivo serotonergic trophic activity. The distribution of serotonergic fibers, determined by immunohistochemistry, in the brains of S100B-knockout mice was quite similar to that of wild-type mice. Furthermore, the content of serotonin and its metabolite 5-hydroxyindole-3-acetic acid in knockout mice was also indistinguishable from those of wild-type mice. Our findings argue against the hypothesis that S100B has a crucial role in neurite extension of serotonergic neurons.
MeSH terms
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Animals
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Axons / metabolism
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Axons / ultrastructure
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Brain / cytology
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Brain / embryology
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Brain / growth & development
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Calcium-Binding Proteins / deficiency*
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Calcium-Binding Proteins / genetics
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Carrier Proteins / metabolism
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Cell Differentiation / physiology*
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Female
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Hydroxyindoleacetic Acid / metabolism
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Immunohistochemistry
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Male
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Membrane Glycoproteins / metabolism
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Membrane Transport Proteins*
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Mice
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Mice, Knockout
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Nerve Growth Factors / deficiency*
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Nerve Growth Factors / genetics
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Nerve Tissue Proteins*
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Neurons / cytology
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Neurons / metabolism*
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Raphe Nuclei / cytology
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Raphe Nuclei / embryology*
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Raphe Nuclei / growth & development*
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S100 Calcium Binding Protein beta Subunit
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S100 Proteins*
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Serotonin / metabolism*
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Serotonin Plasma Membrane Transport Proteins
Substances
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Calcium-Binding Proteins
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Carrier Proteins
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Membrane Glycoproteins
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Membrane Transport Proteins
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Nerve Growth Factors
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Nerve Tissue Proteins
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S100 Calcium Binding Protein beta Subunit
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S100 Proteins
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S100b protein, mouse
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Serotonin Plasma Membrane Transport Proteins
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Slc6a4 protein, mouse
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Serotonin
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Hydroxyindoleacetic Acid