Display Settings:

Format

Send to:

Choose Destination
Proc Natl Acad Sci U S A. 2002 Mar 5;99(5):3199-204. Epub 2002 Feb 26.

Neuronal nitric-oxide synthase localization mediated by a ternary complex with synapsin and CAPON.

Author information

  • 1Department of Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

Abstract

The specificity of the reactions of nitric oxide (NO) with its neuronal targets is determined in part by the precise localizations of neuronal NO synthase (nNOS) within the cell. The targeting of nNOS is mediated by adapter proteins that interact with its PDZ domain. Here, we show that the nNOS adapter protein, CAPON, interacts with synapsins I, II, and III through an N-terminal phosphotyrosine-binding domain interaction, which leads to a ternary complex comprising nNOS, CAPON, and synapsin I. The significance of this ternary complex is demonstrated by changes in subcellular localization of nNOS in mice harboring genomic deletions of both synapsin I and synapsin II. These results suggest a mechanism for specific actions of NO at presynaptic sites.

PMID:
11867766
[PubMed - indexed for MEDLINE]
PMCID:
PMC122496
Free PMC Article

Images from this publication.See all images (5)Free text

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk