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Proc Natl Acad Sci U S A. 2002 Mar 5;99(5):2924-9. Epub 2002 Feb 26.

GATA-1 binding sites mapped in the beta-globin locus by using mammalian chIp-chip analysis.

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  • 1Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA.

Abstract

The expression of the beta-like globin genes is intricately regulated by a series of both general and tissue-restricted transcription factors. The hemapoietic lineage-specific transcription factor GATA-1 is important for erythroid differentiation and has been implicated in regulating the expression of the erythroid-specific genes including the genes of the beta-globin locus. In the human erythroleukemic K562 cell line, only one DNA region has been identified previously as a putative site of GATA-1 interaction by in vivo footprinting studies. We mapped GATA-1 binding throughout the beta-globin locus by using chIp-chip analysis of K562 cells. We found that GATA-1 binds in a region encompassing the HS2 core element, as was previously identified, and an additional region of GATA-1 binding upstream of the gammaG gene. This approach will be of general utility for mapping transcription factor binding sites within the beta-globin locus and throughout the genome.

PMID:
11867748
[PubMed - indexed for MEDLINE]
PMCID:
PMC122449
Free PMC Article

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