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    Biotechniques. 2002 Feb;32(2):422-4, 426, 428-30.

    Epitope tagging genomic DNA using a CD-tagging Tn10 minitransposon.

    Telmer CA, Berget PB, Ballou B, Murphy RF, Jarvik JW.

    Carnegie Mellon University, Pittsburgh, PA, USA.

    Here, we describe an efficient system for epitope tagging cloned genes by CD tagging using a mini-Tn10 transposon delivery vector. The system was tested against a lambdaFIX genomic clone of the human nucleolin gene. Transfection of HeLa cells with the tagged gene led to the expression of both the appropriately spliced tagged transcript and the appropriately localized tagged protein.

    PMID: 11848418 [PubMed - indexed for MEDLINE]

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