Structure. 2002 Feb;10(2):147-51.

Small-scale batch crystallization of proteins revisited: an underutilized way to grow large protein crystals.

Source

Department of Biochemistry, University of Wisconsin, Madison, Madison, WI 53705, USA. ivan_rayment@biochem.wisc.edu

Abstract

Growth of high-quality crystals is a major obstacle in many structural investigations. In recent years, the techniques for screening crystals have improved dramatically, whereas the methods for obtaining large crystals have progressed more slowly. This is an important issue since, although many structures can be solved from small crystals with synchrotron radiation, it is far easier to solve and refine structures when strong data is recorded from large crystals. In an effort to improve the size of crystals, a strategy for a small-scale batch method has been developed that in many cases yields far larger crystals than attainable by vapor diffusion.

PMID:: 11839300; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

Research Support, U.S. Gov't, P.H.S.

MeSH Terms

Substances

Grant Support

LinkOut - more resources

Full Text Sources

Other Literature Sources

Supplemental Content

Save items

Related citations in PubMed

Crystallization of soluble proteins in vapor diffusion for x-ray crystallography. [Nat Protoc. 2007]
Crystallization of soluble proteins in vapor diffusion for x-ray crystallography.
Benvenuti M, Mangani S. Nat Protoc. 2007; 2(7):1633-51.
Review Use of detergents in two-dimensional crystallization of membrane proteins. [Biochim Biophys Acta. 2000]
Review Use of detergents in two-dimensional crystallization of membrane proteins.
Rigaud J, Chami M, Lambert O, Levy D, Ranck J. Biochim Biophys Acta. 2000 Nov 23; 1508(1-2):112-28.
Protein crystallization in restricted geometry: advancing old ideas for modern times in structural proteomics. [Methods Mol Biol. 2008]
Protein crystallization in restricted geometry: advancing old ideas for modern times in structural proteomics.
Ng JD, Stevens RC, Kuhn P. Methods Mol Biol. 2008; 426:363-76.
Preparation of protein nanocrystals and their characterization by solid state NMR. [J Magn Reson. 2003]
Preparation of protein nanocrystals and their characterization by solid state NMR.
Martin RW, Zilm KW. J Magn Reson. 2003 Nov; 165(1):162-74.
Review Effects of a microgravity environment on the crystallization of biological macromolecules. [Microgravity Sci Technol. 1993]
Review Effects of a microgravity environment on the crystallization of biological macromolecules.
McPherson A. Microgravity Sci Technol. 1993 Jun; 6(2):101-9.

See reviews... See all...

Cited by 6 PubMed Central articles

Improving Protein Crystal Quality by the Without-Oil Microbatch Method: Crystallization and Preliminary X-ray Diffraction Analysis of Glutathione Synthetase from Pseudoalteromonas haloplanktis. [Int J Mol Sci. 2011]
Improving Protein Crystal Quality by the Without-Oil Microbatch Method: Crystallization and Preliminary X-ray Diffraction Analysis of Glutathione Synthetase from Pseudoalteromonas haloplanktis.
Merlino A, Russo Krauss I, Albino A, Pica A, Vergara A, Masullo M, De Vendittis E, Sica F. Int J Mol Sci. 2011; 12(9):6312-9. Epub 2011 Sep 23.
What's in a drop? Correlating observations and outcomes to guide macromolecular crystallization experiments. [Cryst Growth Des. 2011]
What's in a drop? Correlating observations and outcomes to guide macromolecular crystallization experiments.
Luft JR, Wolfley JR, Snell EH. Cryst Growth Des. 2011 Mar 2; 11(3):651-663.
Phosphate coordination and movement of DNA in the Tn5 synaptic complex: role of the (R)YREK motif. [Nucleic Acids Res. 2008]
Phosphate coordination and movement of DNA in the Tn5 synaptic complex: role of the (R)YREK motif.
Klenchin VA, Czyz A, Goryshin IY, Gradman R, Lovell S, Rayment I, Reznikoff WS. Nucleic Acids Res. 2008 Oct; 36(18):5855-62. Epub 2008 Sep 12.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Small-scale batch crystallization of proteins revisited: an underutilized way to...
Small-scale batch crystallization of proteins revisited: an underutilized way to grow large protein crystals.
Structure. 2002 Feb ;10(2):147-51.

PubMed
A Gondwanan origin of passerine birds supported by DNA sequences of the endemic ...
A Gondwanan origin of passerine birds supported by DNA sequences of the endemic New Zealand wrens.
Proc Biol Sci. 2002 Feb 7 ;269(1488):235-41.

PubMed
RNA interference: advances and questions.
RNA interference: advances and questions.
Philos Trans R Soc Lond B Biol Sci. 2002 Jan 29 ;357(1417):65-70.

PubMed
Professional development for dentists: patterns and their implications.
Professional development for dentists: patterns and their implications.
Aust Dent J. 2001 Dec ;46(4):289-97.

PubMed
Influence of weather conditions on rheumatic pain.
Influence of weather conditions on rheumatic pain.
J Rheumatol. 2002 Feb ;29(2):335-8.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: