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Science. 2002 Mar 1;295(5560):1702-5. Epub 2002 Feb 7.

Protein nanoarrays generated by dip-pen nanolithography.

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  • 1Northwestern University, Department of Chemistry and Center for Nanofabrication and Molecular Self-Assembly, 2145 Sheridan Road, Evanston, IL 60208, USA.

Abstract

Dip-pen nanolithography was used to construct arrays of proteins with 100- to 350-nanometer features. These nanoarrays exhibit almost no detectable nonspecific binding of proteins to their passivated portions even in complex mixtures of proteins, and therefore provide the opportunity to study a variety of surface-mediated biological recognition processes. For example, reactions involving the protein features and antigens in complex solutions can be screened easily by atomic force microscopy. As further proof-of-concept, these arrays were used to study cellular adhesion at the submicrometer scale.

PMID:
11834780
[PubMed - indexed for MEDLINE]
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