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Acta Biochim Pol. 2001;48(3):657-61.

Electroporated intact BY-2 tobacco culture cells as a model of transient expression study.

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  • 1Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznań, Poland.

Abstract

Transfer of foreign genes into plant cells can be accomplished by several methods: agrobacterium-mediated, microinjection, biolistic particle bombardment and electroporation. The last one is frequently used for transfection of plant protoplasts for transient gene expression. Electroporation is a simple procedure and allows transfecting a large number of cells at one time. Square wave-modulated porators are the most efficient for introducing expression cassettes into plant protoplasts. Based on a protocol developed by Wu & Feng (Plant Cell Reports, 1999, 18, 381-386), we optimized conditions for transfection of intact Nicotiana tabacum BY-2 cells using square wave-modulated electroporator. To simplify screening for transfected gene expression we used constructs with a GFP marker gene.

PMID:
11833774
[PubMed - indexed for MEDLINE]
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