Enhanced podophyllotoxin production from Agrobacterium rhizogenes transformed cultures of Podophyllum hexandrum

A Giri; C C Giri; V Dhingra; M L Narasu

doi:10.1080/10575630108041286

Enhanced podophyllotoxin production from Agrobacterium rhizogenes transformed cultures of Podophyllum hexandrum

Nat Prod Lett. 2001;15(4):229-35. doi: 10.1080/10575630108041286.

Authors

A Giri¹, C C Giri, V Dhingra, M L Narasu

Affiliation

¹ Centre for Biotechnology, Jawaharlal Nehru Technological University, Mahaveer Marg, Hyderabad, India.

PMID: 11833617
DOI: 10.1080/10575630108041286

Abstract

Podophyllotoxin, a potent chemotherapeutic agent is obtained from Podophyllum hexandrum Royle. Embryos of P. hexandrum were transformed using different strains of Agrobacterium rhizogenes viz. A4, 15834, K599. Transformed nature of the calli was ascertained and the cultures were further maintained as individual clones. HPLG analysis of transformed cultures depicted a three-fold increase in podophyllotoxin content in comparison to controls.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Transformed / metabolism
Cells, Cultured / metabolism*
Chromatography, High Pressure Liquid
Guanine / analogs & derivatives
Guanine / chemistry
Guanine / isolation & purification*
Guanine / pharmacology
New Zealand
Plants, Medicinal / chemistry*
Podophyllotoxin / biosynthesis*
Podophyllotoxin / chemistry
Podophyllotoxin / metabolism
Podophyllum / cytology
Podophyllum / metabolism*
Rhizobium / metabolism*
Rhizome / chemistry
Seeds / chemistry
Time Factors
Transformation, Genetic

Substances

N(2),N(2),7-trimethylguanine
Guanine
Podophyllotoxin