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J Clin Microbiol. 2002 Feb;40(2):633-6.

Selective amplification of tyv (rfbE), prt (rfbS), viaB, and fliC genes by multiplex PCR for identification of Salmonella enterica serovars Typhi and Paratyphi A.

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  • 1Department of Bacteriology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan. hirosek@nih.go.jp

Abstract

The PCR primers for O, H, and Vi antigen genes, tyv (rfbE), prt (rfbS), fliC-d, fliC-a, and viaB, were designed and used for the rapid identification of Salmonella enterica serovars Typhi and Paratyphi A with multiplex PCR. The results showed that all the clinical isolates examined of Salmonella serovars Typhi and Paratyphi A were accurately identified by this assay.

PMID:
11825983
[PubMed - indexed for MEDLINE]
PMCID:
PMC153373
Free PMC Article
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