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Biochim Biophys Acta. 2002 Jan 31;1594(1):109-14.

Purification, crystallisation and X-ray diffraction study of fully functional laccases from two ligninolytic fungi.

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  • 1Institute of Biochemistry, Swiss Federal Institute of Technology (ETH), Universitätstrasse 16, CH-8092 Zürich, Switzerland.

Abstract

Laccase isozymes from the white-rot basidiomycete fungi Trametes versicolor and Pycnoporus cinnabarinus were purified to apparent iso-electric homogeneity and crystallised. T. versicolor laccase crystallises in two crystal forms, both with the orthorhombic space group P2(1)2(1)2(1), which diffract to 1.9 and 2.95 A resolution, respectively. The crystals of P. cinnabarinus laccase belong to the monoclinic space group C2 and diffract to at least 2.2 A resolution. All the laccase crystals are suitable for X-ray structure determination and contain a full complement of copper ions.

PMID:
11825613
[PubMed - indexed for MEDLINE]
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