C-reactive protein induces signaling through Fc gamma RIIa on HL-60 granulocytes

J Immunol. 2002 Feb 1;168(3):1413-8. doi: 10.4049/jimmunol.168.3.1413.

Abstract

Human C-reactive protein (CRP) at acute phase levels of 10-200 microg/ml triggered the phosphorylation of FcgammaRIIa, Syk kinase, and phospholipase Cgamma2 in granulocytic HL-60 cells. CRP also stimulated translocation to the membrane of both phospholipase Cgamma2 and phosphatidylinositol-3-kinase. The signaling response triggered by CRP was a rapid, early event with kinetics similar to the response elicited by human IgG. Both soluble-aggregated CRP and monomeric CRP cross-linked FcgammaRII to generate a signal of the same intensity. The results are consistent with signaling through the intrinsic immunoreceptor tyrosine-based activation motif of the cytoplasmic domain of FcgammaRIIa, the major CRP-receptor on monocytes and neutrophils that is responsible for CRP-mediated phagocytosis. The signaling events driven by CRP have the potential to regulate infiltrating neutrophil activities.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, CD / metabolism
  • Antigens, CD / physiology*
  • C-Reactive Protein / physiology*
  • Calcium / metabolism
  • Calcium Signaling / immunology
  • Cell Membrane / enzymology
  • Cell Membrane / metabolism
  • Enzyme Precursors / metabolism
  • Granulocytes / enzymology
  • Granulocytes / immunology*
  • Granulocytes / metabolism*
  • HL-60 Cells
  • Humans
  • Intracellular Fluid / metabolism
  • Intracellular Signaling Peptides and Proteins
  • Isoenzymes / metabolism
  • Phosphatidylinositol 3-Kinases / metabolism
  • Phospholipase C gamma
  • Phosphorylation
  • Protein-Tyrosine Kinases / metabolism
  • Receptors, IgG / metabolism
  • Receptors, IgG / physiology*
  • Signal Transduction / immunology*
  • Syk Kinase
  • Type C Phospholipases / metabolism
  • src-Family Kinases / metabolism

Substances

  • Antigens, CD
  • Enzyme Precursors
  • Fc gamma receptor IIA
  • Intracellular Signaling Peptides and Proteins
  • Isoenzymes
  • Receptors, IgG
  • C-Reactive Protein
  • Phosphatidylinositol 3-Kinases
  • Protein-Tyrosine Kinases
  • SYK protein, human
  • Syk Kinase
  • src-Family Kinases
  • Type C Phospholipases
  • Phospholipase C gamma
  • Calcium