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Nat Genet. 2002 Feb;30(2):141-2. Epub 2002 Jan 14.

Identification of foreign gene sequences by transcript filtering against the human genome.

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  • 1Department of Adult Oncology, Dana-Farber Cancer Institute, 44 Binney Street, Boston, Massachusetts 02115, USA.


We have developed a computational subtraction approach to detect microbial causes for putative infectious diseases by filtering a set of human tissue-derived sequences against the human genome. We demonstrate the potential of this method by identifying sequences from known pathogens in established expressed-sequence tag libraries.

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