Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Transfusion. 2001 Dec;41(12):1500-4.

High-titer screening PCR: a successful strategy for reducing the parvovirus B19 load in plasma pools for fractionation.

Author information

  • 1Aventis Behring, and Aventis Bio-Services Europe, Marburg, Germany. Thomas.Weimer@aventis.com

Abstract

BACKGROUND:

Human parvovirus B19 (B19) is regarded as a potential risk factor for certain patient populations receiving plasma components.

STUDY DESIGN AND METHODS:

The prevalence of B19 was determined in a limited plasma donor population. Conditions for high-titer screening PCR were designed to allow the removal of plasma donations in the acute phase of infection with virus loads >or=10(7) genome equivalents per milliliter before manufacturing. Antithrombin III lots originating from screened plasma were compared to lots originating from untested plasma with respect to their B19 DNA load by a sensitive PCR assay.

RESULTS:

B19 was shown to have a prevalence of about 1 per 800 plasma donations. Only a minority (1/8000) of occurrences were in the acute phase of infection. Removing plasma units with high virus load as determined by high-titer screening PCR significantly decreased peak virus loads of plasma pools for fractionation. Together with a virus-removal capacity of 10.4 log(10) of the manufacturing process, this screening resulted in a final antithrombin III product that was nonreactive for B19 on PCR.

CONCLUSION:

Combining the strategy of high-titer screening PCR with the virus reduction capacity of the manufacturing process considerably increased the margin of B19 virus safety of antithrombin III. This strategy should have positive impact on other plasma components as well.

PMID:
11778063
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Blackwell Publishing
    Loading ...
    Write to the Help Desk