Abstract
SpoIIIE mediates postseptational chromosome partitioning in Bacillus subtilis, but the mechanism controlling the direction of DNA transfer remains obscure. Here, we demonstrated that SpoIIIE acts as a DNA exporter: When SpoIIIE was synthesized in the larger of the two cells necessary for sporulation, the mother cell, DNA was translocated into the smaller forespore; however, when it was synthesized in the forespore, DNA was translocated into the mother cell. Furthermore, the DNA-tracking domain of SpoIIIE inhibited SpoIIIE complex assembly in the forespore. Thus, during sporulation, chromosome partitioning is controlled by the preferential assembly of SpoIIIE in one daughter cell.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacillus subtilis / genetics
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Bacillus subtilis / metabolism
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Bacillus subtilis / physiology*
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Chromosomes, Bacterial / metabolism*
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DNA, Bacterial / metabolism*
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Green Fluorescent Proteins
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Luminescent Proteins
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Promoter Regions, Genetic
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Protein Structure, Tertiary
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Recombinant Fusion Proteins / metabolism
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Sigma Factor*
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Spores, Bacterial / genetics
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Spores, Bacterial / metabolism
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Spores, Bacterial / physiology*
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Transcription Factors*
Substances
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Bacterial Proteins
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DNA, Bacterial
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Luminescent Proteins
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Recombinant Fusion Proteins
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Sigma Factor
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Transcription Factors
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spoIIR protein, Bacillus subtilis
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spore-specific proteins, Bacillus
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Green Fluorescent Proteins