Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning

Science. 2002 Feb 8;295(5557):1089-92. doi: 10.1126/science.1068228. Epub 2002 Jan 3.

Abstract

The presence of galactose alpha-1,3-galactose residues on the surface of pig cells is a major obstacle to successful xenotransplantation. Here, we report the production of four live pigs in which one allele of the alpha-1,3-galactosyltransferase locus has been knocked out. These pigs were produced by nuclear transfer technology; clonal fetal fibroblast cell lines were used as nuclear donors for embryos reconstructed with enucleated pig oocytes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Animals
  • Animals, Genetically Modified*
  • Cell Line
  • Cloning, Organism*
  • Embryo Transfer
  • Female
  • Fetus
  • Fibroblasts
  • Galactosyltransferases / genetics*
  • Gene Targeting*
  • Genetic Vectors
  • Male
  • Mutagenesis, Insertional
  • Nuclear Transfer Techniques
  • Pregnancy
  • Recombination, Genetic
  • Swine
  • Swine, Miniature / embryology
  • Swine, Miniature / genetics*
  • Transfection

Substances

  • Galactosyltransferases
  • N-acetyllactosaminide alpha-1,3-galactosyltransferase