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Traffic. 2001 Nov;2(11):764-74.

Total internal reflection fluorescence microscopy in cell biology.

Author information

  • Department of Physics & Biophysics Research Division, University of Michigan, Ann Arbor, MI 48109, USA. daxelrod@umich.edu

Abstract

Key events in cellular trafficking occur at the cell surface, and it is desirable to visualize these events without interference from other regions deeper within. This review describes a microscopy technique based on total internal reflection fluorescence which is well suited for optical sectioning at cell-substrate regions with an unusually thin region of fluorescence excitation. The technique has many other applications as well, most notably for studying biochemical kinetics and single biomolecule dynamics at surfaces. A brief summary of these applications is provided, followed by presentations of the physical basis for the technique and the various ways to implement total internal reflection fluorescence in a standard fluorescence microscope.

PMID:
11733042
[PubMed - indexed for MEDLINE]
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