Biotechniques. 2001 Nov;31(5):1106-16, 1118, 1120-1.

DNA probes using fluorescence resonance energy transfer (FRET): designs and applications.

Source

Baylor College of Medicine, Houston, TX, USA. vdidenko@bcm.tmc.edu

Abstract

Fluorescence resonance energy transfer (FRET) is widely used in biomedical research as a reporter method. Oligonucleotides with a DNA backbone and one or several chromophore tags have found multiple applications as FRET probes. They are especially advantageous for the real-time monitoring of biochemical reactions and in vivo studies. This paper reviews the design and applications of various DNA-based probes that use FRET The approaches used in the design of new DNA FRET probes are discussed.

Comment in

Early shape-shifting FRET probe. [Biotechniques. 2002]
Early shape-shifting FRET probe.Didenko VV. Biotechniques. 2002 Feb; 32(2):272.
Novel energy transfer fluorescence labeling cassette. [Biotechniques. 2002]
Novel energy transfer fluorescence labeling cassette.Medintz IL, Berti L, Mathies RA. Biotechniques. 2002 Feb; 32(2):270, 272.

PMID:: 11730017; [PubMed - indexed for MEDLINE]
PMCID:: PMC1941713

Free PMC Article

Images from this publication.See all images (4)Free text

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Grant Support

R03 AG022664-01/AG/NIA NIH HHS/United States

LinkOut - more resources

Full Text Sources

Other Literature Sources

Supplemental Content

Save items

Click here to read article using PubReader

Related citations in PubMed

iFRET: an improved fluorescence system for DNA-melting analysis. [Genome Res. 2002]
iFRET: an improved fluorescence system for DNA-melting analysis.
Howell WM, Jobs M, Brookes AJ. Genome Res. 2002 Sep; 12(9):1401-7.
The use of real-time PCR and fluorogenic probes for rapid and accurate genotyping of newborn mice. [Mol Cell Probes. 2001]
The use of real-time PCR and fluorogenic probes for rapid and accurate genotyping of newborn mice.
Hnatyszyn HJ, Podack ER, Young AK, Seivright R, Spruill G, Kraus G. Mol Cell Probes. 2001 Jun; 15(3):169-75.
Determination of interactions between structured nucleic acids by fluorescence resonance energy transfer (FRET): selection of target sites for functional nucleic acids. [Nucleic Acids Res. 1998]
Determination of interactions between structured nucleic acids by fluorescence resonance energy transfer (FRET): selection of target sites for functional nucleic acids.
Ota N, Hirano K, Warashina M, Andrus A, Mullah B, Hatanaka K, Taira K. Nucleic Acids Res. 1998 Feb 1; 26(3):735-43.
Review Nucleic acid-based fluorescent probes and their analytical potential. [Anal Bioanal Chem. 2011]
Review Nucleic acid-based fluorescent probes and their analytical potential.
Juskowiak B. Anal Bioanal Chem. 2011 Mar; 399(9):3157-76. Epub 2010 Oct 29.
Review Fluorescence resonance energy transfer analysis of escherichia coli RNA polymerase and polymerase-DNA complexes. [Biopolymers. 2001]
Review Fluorescence resonance energy transfer analysis of escherichia coli RNA polymerase and polymerase-DNA complexes.
Heyduk T, Niedziela-Majka A. Biopolymers. 2001-2002; 61(3):201-13.

See reviews... See all...

Cited by 15 PubMed Central articles

Real-time monitoring of RAG-catalyzed DNA cleavage unveils dynamic changes in coding end association with the coding end complex. [Nucleic Acids Res. 2012]
Real-time monitoring of RAG-catalyzed DNA cleavage unveils dynamic changes in coding end association with the coding end complex.
Wang G, Dhar K, Swanson PC, Levitus M, Chang Y. Nucleic Acids Res. 2012 Jul; 40(13):6082-96. Epub 2012 Mar 20.
Allelic clustering and ancestry-dependent frequencies of rs6232, rs6234, and rs6235 PCSK1 SNPs in a Northern Ontario population sample. [J Community Genet. 2012]
Allelic clustering and ancestry-dependent frequencies of rs6232, rs6234, and rs6235 PCSK1 SNPs in a Northern Ontario population sample.
Sirois F, Kaefer N, Currie KA, Chrétien M, Nkongolo KK, Mbikay M. J Community Genet. 2012 Oct; 3(4):319-22. Epub 2012 Feb 4.
Multiplex real-time PCR melting curve assay to detect drug-resistant mutations of Mycobacterium tuberculosis. [J Clin Microbiol. 2011]
Multiplex real-time PCR melting curve assay to detect drug-resistant mutations of Mycobacterium tuberculosis.
Luo T, Jiang L, Sun W, Fu G, Mei J, Gao Q. J Clin Microbiol. 2011 Sep; 49(9):3132-8. Epub 2011 Jul 13.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

DNA probes using fluorescence resonance energy transfer (FRET): designs and appl...
DNA probes using fluorescence resonance energy transfer (FRET): designs and applications.
Biotechniques. 2001 Nov ;31(5):1106-16, 1118, 1120-1.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: