Biotechniques. 2001 Nov;31(5):1036, 1038, 1040-1.

Cre stoplight: a red/green fluorescent reporter of Cre recombinase expression in living cells.

Source

Yale University School of Medicine, New Haven, CT 06520, USA.

Abstract

The Cre/lox system is a powerful genetic tool with which to manipulate the genome. Here, we describe the development of a simple reporter system for Cre recombinase, called the Cre Stoplight. In the absence of Cre, the red fluorescent protein is expressed; when Cre catalyzes a recombination event, the green fluorescent protein is produced. Testing this system in transiently transfected cells showed that it produced robust signals (90% of the cells converted from red to green) when equal amounts of the plasmids encoding Cre recombinase and the Cre Stoplight were used. A 1:100 ratio of enzyme to reporter plasmid produced similar results, and a 1:10000 ratio was necessary to significantly reduce the number of cells converting to green (1%).

PMID:: 11730010; [PubMed - indexed for MEDLINE]

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EY08362/EY/NEI NIH HHS/United States

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Cre stoplight: a red/green fluorescent reporter of Cre recombinase expression in living cells.

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Cited by 3 PubMed Central articles

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