Plant Cell Physiol. 2001 Nov;42(11):1274-81.

Characterization of an Arabidopsis cDNA encoding a subunit of serine palmitoyltransferase, the initial enzyme in sphingolipid biosynthesis.

Tamura K, Mitsuhashi N, Hara-Nishimura I, Imai H.

Source

Department of Biology, Graduate School of Natural Science, Konan University, Kobe, 658-8501 Japan.

Abstract

Serine palmitoyltransferase (SPT; EC 2.3.1.50) catalyzes the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine in the first step of de novo sphingolipid biosynthesis. In this study, we describe the cloning and functional characterization of a cDNA from Arabidopsis thaliana encoding the LCB2 subunit of SPT. The Arabidopsis LCB2 (AtLCB2) cDNA contains an open reading frame of 1,467 nucleotides, encoding 489 amino acids. The predicted polypeptide contains three transmembrane helices and a highly conserved motif involved in pyridoxal phosphate binding. Expression of this open reading frame in the Saccharomyces cerevisiae mutant strains defective in SPT activity resulted in the expression of a significant level of sphinganine, suggesting that AtLCB2 cDNA encodes SPT. Southern blot analysis and inspection of the complete Arabidopsis genome sequence database suggest that there is a second LCB2-like gene in Arabidopsis. Expression of a green fluorescent protein (GFP) fusion product in suspension-cultured tobacco BY-2 cells showed that AtLCB2 is localized to the endoplasmic reticulum. AtLCB2 cDNA may be used to study how sphingolipid synthesis is regulated in higher plants.

PMID:: 11726713; [PubMed - indexed for MEDLINE]

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Research Support, Non-U.S. Gov't

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Cloning and characterization of a cDNA encoding serine palmitoyltransferase in Arabidopsis thaliana. [Biochem Soc Trans. 2000]
Cloning and characterization of a cDNA encoding serine palmitoyltransferase in Arabidopsis thaliana.
Tamura K, Nishiura H, Mori J, Imai H. Biochem Soc Trans. 2000 Dec; 28(6):745-7.
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Cited by 3 PubMed Central articles

Sphingolipids in the root play an important role in regulating the leaf ionome in Arabidopsis thaliana. [Plant Cell. 2011]
Sphingolipids in the root play an important role in regulating the leaf ionome in Arabidopsis thaliana.
Chao DY, Gable K, Chen M, Baxter I, Dietrich CR, Cahoon EB, Guerinot ML, Lahner B, Lü S, Markham JE, et al. Plant Cell. 2011 Mar; 23(3):1061-81. Epub 2011 Mar 18.
Serine palmitoyltransferase, a key enzyme for de novo synthesis of sphingolipids, is essential for male gametophyte development in Arabidopsis. [Plant Physiol. 2008]
Serine palmitoyltransferase, a key enzyme for de novo synthesis of sphingolipids, is essential for male gametophyte development in Arabidopsis.
Teng C, Dong H, Shi L, Deng Y, Mu J, Zhang J, Yang X, Zuo J. Plant Physiol. 2008 Mar; 146(3):1322-32. Epub 2008 Jan 24.
The essential nature of sphingolipids in plants as revealed by the functional identification and characterization of the Arabidopsis LCB1 subunit of serine palmitoyltransferase. [Plant Cell. 2006]
The essential nature of sphingolipids in plants as revealed by the functional identification and characterization of the Arabidopsis LCB1 subunit of serine palmitoyltransferase.
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Characterization of an Arabidopsis cDNA encoding a subunit of serine palmitoyltransferase, the initial enzyme in sphingolipid biosynthesis.
Plant Cell Physiol. 2001 Nov ;42(11):1274-81.

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