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Nucleic Acids Res. 2001 Dec 1;29(23):E118.

Signal amplification by rolling circle amplification on DNA microarrays.

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  • 1Molecular Staging Inc., 300 George Street, New Haven, CT 06511, USA. girishn@molecularstaging.com

Abstract

While microarrays hold considerable promise in large-scale biology on account of their massively parallel analytical nature, there is a need for compatible signal amplification procedures to increase sensitivity without loss of multiplexing. Rolling circle amplification (RCA) is a molecular amplification method with the unique property of product localization. This report describes the application of RCA signal amplification for multiplexed, direct detection and quantitation of nucleic acid targets on planar glass and gel-coated microarrays. As few as 150 molecules bound to the surface of microarrays can be detected using RCA. Because of the linear kinetics of RCA, nucleic acid target molecules may be measured with a dynamic range of four orders of magnitude. Consequently, RCA is a promising technology for the direct measurement of nucleic acids on microarrays without the need for a potentially biasing preamplification step.

PMID:
11726701
[PubMed - indexed for MEDLINE]
PMCID:
PMC96711
Free PMC Article

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