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J Clin Microbiol. 2001 Dec;39(12):4514-7.

Identification of Mycobacterium avium subsp. paratuberculosis in biopsy specimens from patients with Crohn's disease identified by in situ hybridization.

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  • 1Dipartimento di Scienze Biomediche, Sezione di Microbiologia Sperimentale e Clinica, Universit√† degli studi di Sassari, 07100 Sassari, Italy.

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  • J Clin Microbiol 2002 Feb;40(2):739. Manuela, M [corrected to Mura, M]; Francesco, T [corrected to Tanda, F]; Amelia, L [corrected to Lissia, A]; Antonello, S [corrected to Solinas, A]; Giovanni, F [corrected to Fadda, G]; Stefania, Z [corrected to Zanetti, S].


Crohn's disease is a chronic inflammatory disease of the gastrointestinal tract of unknown etiology. We report on the presence of cell wall-deficient Mycobacterium avium subsp. paratuberculosis in 35 of 48 paraffin-embedded tissue specimens from 33 patients with Crohn's disease by in situ hybridization with IS900 as a probe.

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