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J Clin Microbiol. 2001 Dec;39(12):4483-6.

Detection of pathogenic Yersinia enterocolitica by a rapid and sensitive duplex PCR assay.

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  • 1National Institute of Public Health and the Environment, Bilthoven, The Netherlands.

Erratum in

  • J Clin Microbiol 2002 Feb;40(2):739.


A duplex PCR assay targeting the ail and 16S rRNA genes of Yersinia enterocolitica was developed to specifically identify pathogenic Y. enterocolitica from pure culture. Validation of the assay was performed with 215 clinical Yersinia strains and 40 strains of other bacterial species. Within an assay time of 4 h, this assay offers a very specific, reliable, and inexpensive alternative to the conventional phenotypic assays used in clinical laboratories to identify pathogenic Y. enterocolitica.

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